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| 一组小麦秸秆好氧分解菌复合系的酶学特性研究 |
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董玉玲1,2, 朱万斌2,3, 郭鹏2, 王小芬2,3, 张利莉1, 崔宗均2,3
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| (1.塔里木大学 新疆兵团塔里木盆地生物资源保护利用重点实验室,新疆 843300;2.中国农业大学 农学与生物技术学院,北京 100193;3.中国农业大学 生物质工程中心,北京 100193) |
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| 摘要: |
| 为加快小麦秸秆木质纤维素酶解,提高小麦秸秆资源的利用率,探讨了一组小麦秸秆好氧分解菌复合系的酶活表达特性。该复合系能高效的分解秸秆,对纤维素的分解率达到80.0%,利用DNS法测定该复合系分泌的酶的酶活性。研究表明,在复合系分解的0~10 d内,0.78 g纤维素被分解,0.16 g半纤维素被分解;复合系分泌的酶是一组能够降解不同底物的酶复合系;复合系的最高纤维素酶活性(内切酶、外切酶、β-糖苷酶和总纤维素酶)为0.17 U/mL;最高木聚糖酶活性出现在第2 天,其数值达到2.82 U/mL;最适木聚糖酶反应温度为50 ℃,最高耐受温度是60 ℃,最适木聚糖酶反应pH为7,pH≤5对酶活性产生强烈抑制;酶反应时间6~20 min时,酶活性急剧下降,以后至酶反应时间120 min时,酶活性下降缓慢。第3 天的0.5 mL离心上清液在最适酶反应条件下酶解2 h后,木聚糖底物被酶解6.76 mg,转化率为33.8%。 |
| 关键词: 小麦秸秆 复合系 好氧分解 木聚糖酶 |
| DOI：10.11841/j.issn.1007-4333.2010.01.001 |
| 投稿时间：2009-05-10 |
| 基金项目:国家"十一五"科技支撑计划重大项目(2006BAD07A01); 教育部博士点新教师(20070019055); 国家自然科学基金青年科学基金(30800672)第一作者:董玉玲,硕士研究生,E-mail:dongyuling80@126.com通讯作者:崔宗均,教授,主要研究方向为生物质资源利用与微生物生态,E-mail:acuizj@cau.edu.cn |
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| Enzymatic activity characteristics of a wheat straw aerobic degradation microbial community |
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DONG Yu-ling1,2, ZHU Wan-bin2,3, GUO Peng2, WANG Xiao-fen2,3, ZHANG Li-li1, CUI Zong-jun2,3
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| (1.Key Lab of Protect and Utilization of Biology Resources in Tarim Basin of Xinjiang Product and Construct,Tarim University,Xinjiang 843300,China;2.College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;3.Center of Biomass Engineering,China Agricultural University,Beijing 100193,China) |
| Abstract: |
| Enzyme activities from a wheat straw aerobic-degradation-microbial community were studied by using DNS method. These enzymes have stable and efficient capacity to decompose 80% of straw cellulose. Cellulose and hemicellulose lost 0.78 g and 0.16 g respectively 10 days after decomposition with these enzymes. The excreted enzymes of microbial community were a group of enzyme complex community with the capacity of zymohydrolyzing different substrates. The maximum cellulase activity was 0.17 U/mL. The maximum xylanase activity was found on the second day with a concentration of 2.82 U/mL. The optimum temperature for xylanase was 50 ℃, while the maximum tolerant temperature was 60 ℃. The optimum pH for xylanase was 7.0. At pH≤5 the xylanase activity was severely inhibited. Xylanase activity dropped quickly 6-20 min after initiation of the enzyme reaction. Afterwards until 120 min, xylanase enzyme activity dropped slowly. Zero point five milliliter centrifugal supernatant on the third day could saccharificate xylan substrate (22.5 mg/0.5 mL) of 6.76 mg with the transfer ratio of 33.8% in two hours under the optimum conditions of xylanase activity. |
| Key words: wheat straw microbial community aerobic degradation xylanase |
