| 摘要: |
| 构建了绵羊朊蛋白的原核表达载体,获得了高纯度的融合表达蛋白。并在热力学因素的作用下,研究了重组绵羊朊蛋白的构象转化。以基因型为ARQ/ARQ蒙古绵羊的血液DNA为模板,利用DNA重组技术,将绵羊朊蛋白正常成熟蛋白基因OvPrP插入表达载体pET30a,在大肠杆菌BL2l(DE3)中高效表达,获得的表达产物以包涵体形式存在,并对其进行纯化和复性。超滤浓缩后浓度约为0.5 mg/mL的OvPrP97-234,进行热力学处理,利用远紫外线圆二色谱(CD)分析热力学处理前、后蛋白的二级结构的变化,同时,对热力学处理前、后的蛋白进行了蛋白酶K抗性的检测,并对其高级结构进行了预测。结果表明:获得的表达产物经SDS-PAGE分析可见分子量为16kD的蛋白条带,Western-blotting的鉴定证实了所获得的蛋白是特异性的朊蛋白。经Jascow32软件分析,测得天然构象的OvPrP97-234的二级结构含量为:α螺旋为28.8%、β折叠为0%、转角和无规卷曲为71.1%,无蛋白酶K的抗性。经过热力学处理之后,OvPrP97-234的二级结构含量为:α螺旋为19.3%,β折叠为36.9%,转角和无规卷曲为43.8%,有一定... |
| 关键词: 绵羊重组朊蛋白 原核表达 构象转化 圆二色谱 热力学因素 |
| DOI：10.11841/j.issn.1007-4333.2009.04.077 |
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| 基金项目:国家自然科学基金资助项目(30871854);;国家科技支撑计划资助项目(2006BAD06A13) |
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| Effect of the thermodynamic factor on conformational conversion of the ovine prion protein in vitro |
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| Abstract: |
| This study obtained fusion protein with high purity of ovine prion protein,and constructed the recombinant prokaryotic expression vector for ovine prion protein gene.It investigated the conformational conversion of ovine prion protein by the effect of the thermal denaturation in vitro.The ovine prion protein gene(Prnp) was expressed in E.coli.by the DNA recombination.The OvPrPC occurred in the sensitive cells(BL2 1) by the denatured inclusion body.After purification and refoldation of the recombinant OvPrPC... |
| Key words: recombinant ovine prion protein prokaryotic expression conformational change circular dichroism spectra thermal denaturation |
