| 摘要: |
| 利用鸟氨酸脱羧酶(ODC)siRNA干涉、ODC过表达、添加特异性ODC抑制剂二氟甲基鸟氨酸(DFMO)处理小鼠子宫内膜上皮细胞和基质细胞,采用实时定量PCR对多胺代谢相关酶类mRNA水平进行检测。结果显示:在子宫内膜上皮和基质细胞中,ODC siRNA转染后可导致SSAT mRNA水平下降为对照组的71.78%和54.36%(P<0.01),SAMDC mRNA水平上升为对照组的1.666 6倍和3.036 6倍(P<0.01);ODC过表达造成SSAT水平为对照组的1.921 4和2.852 7倍(P<0.01),AZⅠmRNA水平为对照组的1.962 1和3.073 8倍(P<0.01);利用DFMO处理体外分离的上皮细胞和基质细胞,SSAT mRNA水平为对照组的1.626 7和1.773 3倍(P<0.01),SAMDC mRNA水平为对照组的1.630 0和1.730 0倍(P<0.01),AZⅠmRNA水平为对照组的71.00%和76.67%(P<0.01);DFMO处理后上皮细胞和基质细胞数目(31.00±3.00和43.67±7.51)和活力(0.26±0.03和0.36±0.05)都显著下... |
| 关键词: 小鼠 子宫内膜细胞 鸟氨酸脱羧酶 二氟甲基鸟氨酸 多胺代谢相关基因 |
| DOI：10.11841/j.issn.1007-4333.2009.03.051 |
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| 基金项目:国家“973”计划项目(2006CB504005) |
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| Regulation of polyamine-related genes in mice endometrial cell |
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| Abstract: |
| The aim of this study was to regulate the polyamine-related genes transcription in mouse endometrial cell by ornithine decarboxylase(ODC) siRNA,ODC over-expression vector and ODC specific inhibitor-difluoromethylornithine(DFMO),and polyamine-related genes were detected by real-time PCR.Results showed that ODC siRNA led to an obvious increase of S-adenosylmethionine decarboxylase(SAMDC)(1.666 6 times and 3.036 6 times compared with control) and decrease of spermidine/spermine N1-acetyltransferase(SSAT) trans... |
| Key words: mice endometrial cell ornithine decarboxylase difluoromethylornithine polyamine-related gene |
