| 摘要: |
| 建立了猪饲料中螺旋霉素、替米考星和泰乐菌素3种大环内酯类药物的HPCE检测方法.确定了该过程中缓冲溶液的pH、缓冲溶液类型及离子强度等电泳条件,同时对毛细管长度、内径及分离电压等仪器参数进行优化,得到分离的最优条件:在毛细管有效长度50 cm、内径75 μm,分离电压25 kV,40 mmol/L磷酸氢二钠-20mmol/L柠檬酸(pH2.5)缓冲液条件下,3种药物在15 min内得到完全分离.3种药物在10～100 μg/g范围内线性良好,R>0.999 3;饲料中螺旋霉素检测限为3.0 μg/g,替米考星检测限为5.0μg/g,泰乐菌素检测限为8.0 μg/g,添加回收率在74.5%～87.4%,方法的日内变异系数<8.1%,日间变异系数<7.0%.本方法简单快速,分离环境为水相,经济又环保,适用于大量样本的筛选. |
| 关键词: 高效毛细管电泳 螺旋霉素 替米考星 泰乐菌素 饲料检测 |
| DOI：10.11841/j.issn.1007-4333.2009.01.021 |
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| 基金项目:国家科技支撑计划? |
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| Determination of macrolides in feeds by capillary electrophoresis |
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| Abstract: |
| A HPCE method has been developed for the separation of three macrolide antibiotics,Spiramycin(SPM),Tilmicosin(TIL) and Tylosin(TYL) in swine feeds.A systematic approach was used to maximise analyte differential electrophoretic mobility by manipulating electrolyte pH,composition and molarity.In addition,some instrumental parameters such as capillary length and diameter and applied voltage were varied.The analysis was performed in a 40 mmol/L Na2HPO4-20 mmol/L Citric acid buffer(pH 2.5) and an applied voltage... |
| Key words: HPCE Spiramycin Tilmicosin Tylosin feeds determination |
