| 摘要: |
| 分析不同品种牛的瘤胃微生物群落结构的差异,构建了1个鲁西肉牛瘤胃细菌16S rDNA文库,并从公共数据库中搜集来自荷斯坦奶牛、晋南牛、牦牛、黄牛和Hanwoo的9个16S rDNA文库,对序列多样性和文库组成等进行比较分析.结果发现:鲁西内牛瘤胃细菌16S rDNA文库共有122个克隆,可分为109个操作分类单元.文库中91%的克隆来自未培养细菌,拟杆菌(Bacteroidales)和梭菌(Clostridiales)是其中的优势菌祥.文库的定性和定量比较表明饲喂不同日粮的5个荷斯坦奶牛16S rDNA文库组成类似,而与鲁西肉牛等亚洲品种的文库存在显著差异.结果表明,牛的品种是瘤胃细菌群落差异的重要因素,对瘤胃细菌群落组成的影响大于日粮或其他因素. |
| 关键词: 牛 瘤胃细菌群落 多样性 |
| DOI：10.11841/j.issn.1007-4333.2009.01.003 |
|
| 基金项目:国家奶业科技支撑计划，动物营养学国家重点实验室自主研究课题? |
|
| Comparative analysis of rumen microbial communities in six species of cattle |
|
|
|
| Abstract: |
| To improve our understanding of factors that may influence the structure of rumen microbial community,we constructed bacterial 16S rDNA clone libraries from Chinese Nooxi steers,extracted nine 16S rDNA libraries from the public database and then subjected them to ecological statistical analysis.Results showed that the Nooxi 16S rDNA library contained 122 clones,covered a reasonable fraction of rumen bacterial community and could be used for library comparison.Principal component analysis and nonparametric e... |
| Key words: 16S rDNA |
