| 摘要: |
| 探讨近红外漫反射光谱技术(NIRS)测定新鲜青贮饲料化学成分的可行性。在液氮(-196℃)冷冻下对119份具有代表性的青贮饲料样品进行粉碎处理,利用传统分析方法测得其化学成分含量,同时利用近红外光谱仪扫描得到其近红外光谱,采用改良偏最小二乘回归方法(MPLS)建立其近红外定量分析校正模型。结果表明:干物质(DM)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)和粗灰分(CA)的相对分析误差RPD值(SD/SECV)均大于3,校正模型的外部验证决定系数R2v均大于0.85;粗蛋白(CP)、酸性洗涤木质素(ADL)和可溶性碳水化合物(WSC)的RPD值均大于2,其R2v分别为:0.82、0.65和0.71。近红外漫反射光谱法可以很好的测定新鲜青贮饲料中的DM、NDF、ADF和CA含量;CP含量测定效果较好,需要通过扩大样品CP含量变异范围进一步验证;可粗略估测ADL和WSC含量,测定精度有待于提高。 |
| 关键词: 近红外漫反射光谱(NIRS) 快速测定 新鲜青贮饲料 化学成分 定量分析 |
| DOI：10.11841/j.issn.1007-4333.2007.06.132 |
| 投稿时间：2007-04-16 |
| 基金项目:国家科技攻关项目 |
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| Rapid measurement for chemical compositions of fresh silage by near-infrared reflectance spectroscopy |
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| Abstract: |
| In order to study the feasibility of analysis of chemical compositions of fresh silage using near-infrared reflectance spectroscopy(NIRS),which is different from the pretreatment of comminuting after drying process as conventional means, a representative population of 119 silage samples was grounded under the protection of liquid nitrogen.Using the traditional methods to analysis chemical composition of the silage samples and using the spectrometer to scan their spectrums at the same time.All the NIRS calibrations for the fresh silage were developed by means of Modified Partial Least-Squares(MPLS) regression.Results showed that the RPD(SD/SECV)values of Dry Matter(DM),Neutral Detergent Fiber(NDF), Acid Detergent Fiber(ADF) and Crude Ash(CA) were all greater than 3.And the correlation coefficient of validation(R~2_v) of the parameters were all greater than 0.85.The RPD of the parameters for Crude Protein(CP),Acid Detergent Lignin(ADL) and Water Soluble Carbohydrate(WSC) were all greater than 2 and the R~2_v of them were 0.82,0.65 and 0.71 respectively.We concluded that NIRS could provide accurate prediction of a wide range of chemical compositions,including the contents of DM,NDF,ADF and CA;and NIRS could provide relatively accurate prediction of CP but extra samples should be collected to enlarge the variation range of CP for further validation.ADL and WSC content in fresh silage can roughly be estimated by NIRS,however,the precisions for them should be improved in future. |
| Key words: near infrared reflectance spectroscopy(NIRS),rapid measurement,fresh silage,chemical composition,quantitative analysis |
