| 摘要: |
| 为构建桃品种核心种质,通过对56份桃(Prunus persica(L.) Batsch.)初级核心种质的形态农艺性状数据(MOR)和SSR等位基因数据的分析,研究了不同聚类取样方法和完全随机取样方法下9种取样比例的遗传多样性指数、保留比例及各频率段等位基因的丢失比例。结果表明:聚类取样的方法优于完全随机取样,并以在80%的取样比例下MOR结合SSR数据聚类取样的效果最好,利用此方案构建的桃品种核心种质共包括45份材料,该核心种质的基因遗传多样性指数最高,保留了初级核心种质100%的形态农艺性状和96.6%的SSR等位基因,在出现频率低于0.05的等位基因中共丢失了2个等位变异,保留了出现频率在0.05~0.10的所有等位基因;利用6个数量性状对所构建的核心种质的代表性检测表明所构建的核心种质很好地代表558份桃原始种质的遗传变异。 |
| 关键词: 桃(Prunus persica (L.) Batsch.) 遗传多样性 保留比例 核心种质 构建 评价 |
| DOI：10.11841/j.issn.1007-4333.2007.05.098 |
| 修订日期：2007-01-25 |
| 基金项目:教育部跨世纪优秀人才培养计划;北京市地区高等学校学科群建设项目 |
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| Establishment and evaluation of the core collection of peach (Prunus persica (L.) Batsch.) cultivars |
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| Abstract: |
| This study was aimed at establishing a core collection based on the analysis of MOR and SSR alleles of the primary core collection data of peach cultivars.The index of genetic diversity,and frequency ratio of retention and loss of alleles were studied in cluster and random samples at 9 sampling ratios.The cluster sampling method preceded random sampling,and cluster sampling of MOR combined with SSR at the sampling ratio of 80% was the best sampling strategy across sampling methods.Based on this sampling strategy,45 accessions were selected as the core collection of peach with the highest index of genetic diversity which retained 100% phenotypic characters and 96.6% alleles of primary core collection.Two alleles were lost at frequencies below 0.05 and all alleles were retained at frequencies between 0.05 and 1.The core collection developed was evaluated by using 6 quantitative traits and the data showed that it can satisfactorily represent the genetic diversity of the original collection of 558 peach accessions.The core collection of peach with a fair representation was established in this study based on pre-determined best sampling strategy. |
| Key words: peach(Prunus persica(L.) Batsch.),genetic diversity,retaining ratio,core collection,establishment,evaluation |
