| 摘要: |
| 为探讨和比较磷酸二酯酶(PDE)3、4和5对小鼠卵母细胞体外自发成熟的影响,将小鼠卵丘卵母细胞复合体(COCs)和裸卵(DOs)分别培养在含有或不含有PDE 3、4和5特异性抑制剂的M-199培养液中。结果表明:PDE3的特异性抑制剂cilostamide和PDE5的特异性抑制剂zaprinast均能显著抑制小鼠COCs和DOs的自发成熟,且其抑制效应不随培养时间的延长而减弱,但却是可逆的;而PDE4的特异性抑制剂rolipram对小鼠COCs和DOs没有影响。上述结果提示,不同亚型的PDE在小鼠卵母细胞减数分裂过程中具有不同的调节作用。 |
| 关键词: 磷酸二酯酶 抑制剂 卵母细胞成熟 小鼠 减数分裂 |
| DOI：10.11841/j.issn.1007-4333.2007.05.094 |
| 修订日期：2007-03-12 |
| 基金项目:国家自然科学基金 |
|
| Effects of the specific inhibitors of phosphodiesterase on oocyte spontaneous meiotic maturation of mouse in vitro |
|
|
|
| Abstract: |
| The objective was to study the effects of the specific inhibitors of PDEs on oocyte spontaneous meiotic maturation of mouse in vitro.Mouse cumulus-oocyte complexes(COCs) and denuded oocytes(DOs) were collected and cultured in a medium supplemented with specific inhibitors of PDEs and the rates of GVBD and PB1 extrusion were observed at different culture periods.The results indicated that both PDE3 inhibitor,cilostamide and PDE5 inhibitor,zaprinast,prevented oocyte spontaneous meiotic maturation.However,the PDE4 inhibitor,rolipram had no effect.The inhibitory effects of both cilostamide and zaprinast did not abate with increasing culture time but was reversed.Collectively,these results suggest that different PDEs have different roles in meiotic maturation of mouse oocytes. |
| Key words: PDE,inhibitors,oocyte maturation,meiotic,mouse |
