| 摘要: |
| 为了进一步研究蒙古黄芪(Astragalus mongholicusBunge)中的蛋白质及其功能,通过金属离子螯合层析、离子交换层析和凝胶过滤得到一种新蛋白(AmPR-10),并对其部分性质进行了研究。AmPR-10在SDS-PAGE上的分子质量为17.2 ku,凝胶过滤层析测得分子质量为32.6 ku,说明其为同源二聚体。糖蛋白染色显示为糖蛋白,总糖含量为13.7%,离子交换色谱法分析其中含有73%阿拉伯糖、15%葡萄糖、微量的果糖和一种未知糖;N-端序列分析结果显示其与病程相关蛋白家族PR-10中黄羽扇豆L1PR-10.1C同源性高达80%。同时,AmPR-10具有核糖核酸酶活性,纯AmPR-10的核糖核酸酶比活性为74.11 U/mg,这点与一些PR-10蛋白类似。 |
| 关键词: 蒙古黄芪 病程相关蛋白PR-10 纯化 核糖核酸酶活性 |
| DOI：10.11841/j.issn.1007-4333.2007.04.076 |
| 修订日期：2006-12-26 |
| 基金项目:北京市自然科学基金 |
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| Purification and characterization of a novel pathogenesis-related class-10 protein from Astragalus mongholicus |
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| Abstract: |
| A novel pathogenesis-related class-10 protein(AmPR-10) from Astragalus mongholicus was purified and characterized.The purified protein appeared as a single band with a molecular mass of 17.2 ku on SDS-PAGE and was eluted from a Superdex-75 column with an apparent molecular weight of 32.6 ku.AmPR-10 was a glycoprotein with a neutral carbohydrate content of 13.7%.The carbohydrate was mainly composed of 73.0% arabinose and 15.0% glucose.N-terminal amino-acid sequence of AmPR-10 was significantly homology to pathogenesis-related proteins from the PR-10 family.The sequence had 80% identity with L1PR10.1C,a PR-10 protein from Lupinus luteus.AmPR-10had ribonuclease activity of 74.11 U/mg protein,as some other PR-10 proteins. |
| Key words: Astragalus mongholicus Bunge,pathogenesis-related class10 proteins,purification,ribonuclease activity |
