| 摘要: |
| 应用乙酰胆碱酯酶(AchE)和NADPH-黄递酶组织化学方法,研究了15日龄、4月龄和12月龄山羊小肠中胆碱能神经元和NO能神经元的数量分布变化。结果显示:1)肌间神经丛和黏膜下神经丛含有丰富的AchE阳性神经元,神经元聚集成神经节,并由节间支连成网状。2)NOS(一氧化氮合成酶)阳性神经元主要分布于肌间神经丛,神经元构成的神经节也连成网状;黏膜下神经丛内NOS阳性神经元稀疏,散在分布。3)小肠肌间神经丛中,12月龄的AchE和NOS阳性神经元总数比15日龄的增加163%和137%,但阳性神经元密度降低39%(AchE)和40%(NOS)。4)比较各肠段,AchE和NOS阳性神经元密度在回肠中最高,但神经元总数以空肠最多。结果表明山羊小肠中分布有丰富的胆碱能和NO能神经元,但黏膜下神经丛中胆碱能神经元多于NO能神经元;随着山羊小肠的发育,胆碱能和NO能神经元的数量逐渐增多而密度却下降。 |
| 关键词: 山羊 肠道神经系统 肌间神经丛 乙酰胆碱酯酶 NADPH-黄递酶 一氧化氮合成酶 |
| DOI：10.11841/j.issn.1007-4333.2007.02.027 |
| 投稿时间：2006-12-22 |
| 基金项目:国家自然科学基金 |
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| Differences of AchE and NOS-positive neuron number and distribution in goat small intestine |
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| Abstract: |
| The changes in distribution and number of acetylcholinesterase(AchE) and nitric oxide synthase(NOS) posi-tive neurons were studied in enteric nervous system of goat small intestine at15days,4months and12months of age using by using histochemistry.The results showed that1) a lot of AchE-positive neurons were observed in submucosa and myenteric plexuses.These neurons gathered in ganglia and formed a net.2) The NOS-positive neurons were main-ly located in the myentrric plexus,but very few NOS-positive neurons were observed in the submucou plexus.3) In myentrric plexus,the total number of AchE and NOS positive neurons was increased163% and137% in12months of age than that of15days of age,respectively.However,the neuron density decreased by39%(AchE) and40%(NOS) in12months of age comparing with that of15days of age.4) The total numbers of AchE and NOS positive neu-rons were the largest in the jejunum,whereas the neuron density was the highest in the ileum,compared with that in other sections of small intestine.In addituion,the number of cholinergic neurons was more than those of NO neurons in the submucou plexus.In the duration of small intestine development,the increase in the cholinergic and NO neurons was accompanied by the decline of NO neuron,while the density. |
| Key words: goat,enteric nervous system,myenteric plexus,acetylcholinesterase,NADPH diaphorase,nitric oxide synthase |
