| 摘要: |
| 利用手性衍生化试剂L-薄荷醇和气相色谱柱前衍生化,将瘤胃液和青贮液中L-和D-乳酸对映体转化为酯类非对映体对,建立同时分析发酵液中乳酸对映体和挥发酸的气相色谱检测方法。样品乳酸对映体的质量浓度在0.05~10.00 mg/mL范围内时,标准曲线的决定系数分别达到R2=0.999 5和R2=0.999 6。乳酸对映体的回收率为96.15%~108.24%,且相对标准偏差小于5%。采用本方法检测瘤胃液和青贮液样品的L-和D-乳酸对映体含量,并且与酶学检测法进行比较,2种方法的测定结果没有显著性差异(P>0.05)。发酵液中挥发酸经衍生化处理,可以与L-和D-乳酸对映体在同一色谱条件下检测,各组分在0.05~5.00 mg/mL范围内线性良好,R2=0.998 2~0.999 4。乳酸对映体和挥发酸的色谱分析在6 min完成。 |
| 关键词: 乳酸对映体,气相色谱法,瘤胃液,青贮液 |
| DOI：10.11841/j.issn.1007-4333.2006.02.039 |
| 投稿时间：2005-08-25 |
| 基金项目:国家自然科学基金资助项目(30270944) |
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| Gas chromatography assay of lactic acid isomers and VFA in rumen and silage fluids by pre-column chiral derivation procedure |
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| Abstract: |
| A novel procedure for determination of lactic acid isomers in the rumen and silage fluid was developed based on pre-column chiral derivation with L-menthol.The determination showed a linear response within the concentration range of 0.05 and 10.00mg/mL for L-and D-lactate isomers with the correlation coefficients of 0.9995 and 0.9996,(respectively).The recovery of each isomer averaged between 96.15% and 108.24%(RSD<5%).A comparison of analytical results of lactate isomers on the rumen and silage fluid established between the enzyme procedure and that in this studyshowed no significant difference(P>0.05).Using gas chromatography conditions described here,6 volatile fatty acids(VFA) including acetate,propionate,iso-butyrate,butyrate,valerate and iso-valerate acids were simultaneously analyzed with both lactate isomers,having high correlation coefficients of 0.998 2 and 0.9994 within the VFA concentration range from 0.05 to 5.00mg/mL.Fermentation fluids were run for 6 min for both the isomers of lactic acid and VFA assay. |
| Key words: lactic acid isomer,gas chromatography,ruminal fluid,silage fluid |
