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番茄采后成熟过程种子和果皮中脱落酸与乙烯代谢的关系
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摘要:
以番茄中杂101为材料,研究采后果实成熟过程种子和果皮中脱落酸(ABA)含量与乙烯生物合成的关系,以及外源ABA及其生物合成抑制剂(fluridone)处理对果实ABA含量和乙烯释放量的影响。结果表明:采后番茄果实成熟过程中.种子的乙烯释放量、1-氨基环丙烷-1-羧酸(ACC)含量和ABA含量均高于同时期果皮;种子和果皮中ABA和ACC含量的峰值都出现在乙烯跃变之前;ACC氧化酶(ACO)活性变化趋势与ABA含量及乙烯释放量的变化趋势相一致;外源ABA处理使果皮和种子中ABA含量显著增加,促进了果实乙烯的生成;fluridone处理则相反。以上证据表明,番茄果实中的ABA通过刺激乙烯的生成促进果实成熟,种子可能通过调控果实内ABA含量和乙烯释放量而影响果实的成熟。
关键词:  番茄果实 种子 成熟 脱落酸 乙烯
DOI:10.11841/j.issn.1007-4333.2005.05.122
投稿时间:2005-01-25
基金项目:基金项目:国家自然科学基金资助项目(30200191);国家“十五”攻关资助项目(2001BA501A109)
Relationship between abscisic acid and ethylene in seed and pericarp during tomato ripening
Abstract:
Endogenous abscisic acid(ABA) content,ethylene production,ACC content,activities of ACC oxidase were measured during ripening process of tomato fruit(Lycopersicon esculentum Mill.cv.Zhongza 101).Although the maximums of ABA content,ACC content and ethylene climacteric peak occurred at different ripening stages,they all increased in seeds before they increased in pericarp tissues.The maximum ABA and ACC contents preceded ethylene production in both the seeds and pericarp.Changes of the activity of ACC oxidase were coincident with that of ethylene production and ABA content.Exogenous ABA treatment increased of ABA content,both in pericarp and seed,and ethylene production,while treatment with fluridone,an inhibitor of ABA synthesis,inhibited them.The results suggest that seeds may impact the process of postharvest tomato fruit ripening by regulating of endogenous ABA content and ethylene production,and ABA regulates fruit ripening by triggering ethylene production.
Key words:  tomato fruit  seed  ripening  abscisic acid  ethylene