| 摘要: |
| 以鸡马立克氏病脾脏淋巴瘤继代细胞系MDCC-MBI(MSB1)为研究对象，用southern和northern斑点分子杂交放射自显影等方法，证明了5-氮胞苷可以阻断MSB1细胞DNA的甲基化，且去甲基化的DNA转录区和部分非转录区有特异增幅的转录产物mRNA，说明这些区基因转录活性上升。用5-氮胞苷处理MSB1细胞使其去甲基化后，该细胞DNA的内切酶Barn HⅠ区域对DNaseⅠ敏感性增高，该片段区的基因表达活性也增高。以上结果提示．DNA的去甲基化可以提高MDV的基因表达，进一步说明MDV DNA的甲基化在抑制MDV基因表达，维持MDV在淋巴瘤细胞株中潜伏感染状态可能起着重要作用。 |
| 关键词: 淋巴瘤细胞 MDV DNA 甲基化 mRNA 5-氮胞苷 |
| DOI：10.11841/j.issn.1007-4333.2004.06.143 |
| 修订日期：2004-06-15 |
| 基金项目: |
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| Effect of DNA demethylation on gene expression from Marek''''s disease virus |
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| Abstract: |
| A variety of Marek's disease virus (MDV) lymphoblastoid cell lines have been established. The MDV lymphoblstoid cell line, MDCC-MB1(MSB1) were treated with 5-azacytidine (5-AZC) and resulted in demethylation of MDV DNA and the increase in mRNA from certain portions of the MDV DNA When MDV DNA was digested with DnaseI, new bands appeared and the increases in mRNA from these portions of the MDV DNA were detected by using southern and northern blotting hybridization. These results suggest MDV DNA transcriptional activity was increased by demethylation, and the methylation of MDV DNA appears to be one of the factors associated with the repression of thanscription of MDV DNA in the lymphoblastoid cell line, MSB1. |
| Key words: lymphoblastoid cell,MDV DNA,demethylation,mRNA sythesis,5-AZC |
