| 摘要: |
| 利用降落PCR的方法。从谷子基因组中获得lcy-b基因。全序列分析表明。其开放阅读框为1782bp，编码的蛋白由594个氨基酸组成。分子质量为67724u。将获得的谷子lcy-b基因序列与已发表的其他植物lcy-b基因序列比较。发现所得到的基因序列与单子叶植物黄水仙的同源性明显高于与双子叶植物的同源性。蛋白质序列比较发现中部和尾部的部分区段内氨基酸同源性较高，且都存在FLYAMP序列和FAD／NAD(P)结合区等植物LCY—B的特征序列。Southern杂交结果表明。lyc-b在基因组中以单拷贝存在。 |
| 关键词: 谷子 类胡萝卜素 生物合成 番茄红素β-环化酶 基因克隆 降落PCR |
| DOI：10.11841/j.issn.1007-4333.2004.01.002 |
| 修订日期：2003-10-29 |
| 基金项目: |
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| Cloning of Lycopene β-cyclase gene related to carotenoid biosynthetic pathway of millet (Setaria italica) |
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| Abstract: |
| Millet lcy-b gene was cloned by the Touch Down-PCR. Sequence analysis showed that the open-reading frame of the cDNA was 1?782 bp and encoded a putative protein with 594 amino acids. The molecular weight of the putative protein was 67?724?u. All of the proteins encoded by lcy-b gene in plants contained a FAD/NAD (P) binding region. The homologue of the obtained gene with monocotyledon daffodil was higher than that of the dicotyledon. And the homologues of the proteins were higher in middle and tail parts. Southern blottiog indicated that lcy-b gene was one copy of the millet genome. |
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