| 摘要: |
| 为了进一步研究链霉菌木聚糖酶的酶学性质，探讨了卷须链霉菌一种木聚糖酶的纯化方法。粗酶液经硫酸铵饱和度为20％～50％的分级沉淀和Q-sephrose FF离子交换柱层析2步纯化，得到一种电泳纯的木聚糖酶。定名为XynB；酶蛋白纯化倍数达到10．0倍，酶活力回收率达到24．0％。SDS-PAGE结果表明，该木聚糖酶的分子质量为22．0ku，属于低分子质量的木聚糖酶。 |
| 关键词: 卷须链霉菌 木聚糖酶B 纯化 硫酸铵沉淀 离子交换层析 |
| DOI：10.11841/j.issn.1007-4333.2003.06.143 |
| 修订日期：2003-05-10 |
| 基金项目:国家“十五”攻关课题 (2 0 0 1BA70 8B0 4 0 6 )，教育部研究重点项目 |
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| Purification of XynB from Streptomyces cirratus |
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| Abstract: |
| To study further the property of xylanases from Streptomyces, purification of a xylanase from Streptomyces cirratus D-10 was investigated. A xylanase, named XynB, was purified by stepwise ammonium sulphate precipitation (20%-50% saturation) and Q-sephrose FF ion exchange chromatography. After two-step treatment, ten-fold purification was obtained with 24.0% activity recovery. The purified XynB showed as a single protein band on SDS-PAGE that molecular weight of the enzyme was estimated to be 22.0 ku by SDS-PAGE, and belonged to one of lower molecular weight xylanases. |
| Key words: Streptomyces cirratus,xylanase,purification,ammonium sulphate precipitation,ion exchange chromatography |
