| 摘要: |
| 为克服大蒜茎尖离体培养增殖率低的问题，对准备切取茎尖组织进行离体培养的大蒜种球以及切取的带有1，2片叶原基的大蒜茎尖组织(外植体)进行了低温处理，结果表明，低温处理促进了大蒜茎尖离体培养时芽的形成及生长。对外植体的低温处理比对种球的低温处理效果好，60d低温处理后，外植体低温处理区的新芽形成数是种球低温处理区的10倍，平均每个茎尖形成新芽22．2个；种球低温处理和外植体低温处理并用时。没有产生累加效应，相反，外植体低温处理的效果，被种球低温处理削弱。 |
| 关键词: 低温处理 大蒜 茎尖组织 离体培养 芽形成 生长 影响因素 增殖率 外植体 |
| DOI：10.11841/j.issn.1007-4333.2003.04.098 |
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| 基金项目: |
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| Effects of low temperature on shoot proliferation and shoot grow in garlic shoot-tip culture |
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| Abstract: |
| In order to overcome the problem of the low frequency of proliferation of garlic shoot-tips culture, the garlic bulbs and the shoot-tips (explants) with 1, 2 leaf primordiums need to be treated under low temperature. The forming and growing of the shoot of garlic shoot-tip cultures were promoted under low temperature. The effect of the treatment on explants was better than that of on bulb. During the same period of 60 days under low temperature, the number of shoots was 10 times as much as that of bulbs. In average, each shoot form 22.2 new shoots. Accumulative effects were not observed as the shoot-tips and bulbs were treated under the same low temperature. On the contrary, the effect on bulbs was weakened than that of on explants. |
| Key words: garlic,shoot-tip culture,explant,low temperature disposing |
