| 摘要: |
| 以杜仲一叶一心期幼叶为材料，提取总BNA，采用RT-PCR技术分离得到498bp核苷酸片段，此核苷酸片段与CenBank中的苹果树肉桂醇脱氢酶(CAD)基因序列有64．1％的同源性，与桉树mRNA CAD有63．9％的同源性。所克隆的cDND编码165个氨基酸残基，其序列与苹果树CAD相应片段有68．5％的同源性，与桉树mRNA CAD相应片段有66．1％的同源性。推测克隆的核香酸片段为杜仲CAD的基因片段。 |
| 关键词: 杜仲 肉桂醇脱氢酶 基因克隆 序列分析 同源性 木质素 |
| DOI：10.11841/j.issn.1007-4333.2003.01.009 |
| 修订日期：2002-06-04 |
| 基金项目:国家自然科学基金资助项目 (39870 6 4 4 )，农业生物技术国家重点实验室基金开放课题资助项目 (9910 0 4 2 ) |
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| Cloning of cDNA encoding cinnamyl alcohol dehydrogenase from Eucommia ulmoides Oliv |
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| Abstract: |
| Cinnamyl alcohol dehydrogenase catalyses the conversion of p hydroxy cinnamaldehydes to the corresponding alcohols and is considered as a key enzyme in lignin biosynthesis. In this study, a cDNA fragment of cinnamyl alcohol dehydrogenase (CAD) was cloned from Eucommia ulmoides Oliv by RT PCR. As deduced from the 498 bp cDNA nucleotide sequence, it presented 68.5% identity with Malus domestica putative CAD and 66.1% identity with E.gunnii mRNA for CAD. These results suggest that the cDNA fragment is from CAD gene of Eucommia ulmoides Oliv. |
| Key words: Eucommia ulmoides Oliv,cinnamyl alcohol dehydrogenase,lignin,DNA sequence |
