| 摘要: |
| 为确定提取老鹳草鞣质的合理生产工艺，及为药物复配提供药理学依据，采用水回流法、水温浸法、丙酮回流法、冷浸搅拌法和乙醇回流法进行了老鹳草鞣质的提取试验；采用MTT方法检测了不同工艺生产的老鹳草鞣质对ConA和LPS刺激鸡外周血中淋巴细胞增殖反应的影响。试验结果表明：水温浸法、丙酮回流法和乙醇回流法鞣质得率和质量分数均较高，考虑经济因素则水温浸法为好。除乙醇回流法外的4种方法提取的老鹳草鞣质在质量浓度较低时对ConA诱导的鸡外周血T淋巴细胞及LPS诱导的B淋巴细胞的增殖均有显著的促进作用。 |
| 关键词: 老鹳草 鞣质 提取工艺 免疫作用 试验研究 中药材 |
| DOI： |
| 修订日期：2002-04-06 |
| 基金项目:国家"十五"科技攻关项目子课题 |
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| Extraction and Immunosuppressive of Tannins From Herba Geranium |
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| Abstract: |
| To determine a reasonalbe extraction technology and provide a reliable pharm acologic base for the complex ratio of the traditional Chinese medicine, extraction of Tannins from Herba Geranium by water reflux, warm water immersion, acetone solution reflux, cold water immersion with stir and alcohol solution reflux were carried out. The effects of Herba Geranium Tannins produced by different extraction technology were investigated on chicken lymphocyte proliferation in vitro with MTT method. The results showed that the extraction yield and content of tannins extracted by warm water immersion, acetone solution reflux and alcohol solution reflux were all higher, the method of warm water immersion was better on the basis of cost. Except the method of alcohol solution reflux, the tannins extracted by the other 4 methods increased markedly the ConA and LPS induced prolixferation of T and B lymphocytes respectively under low concentration. |
| Key words: Herba Geranium,tannins,extraction,immunosuppressive effect |
