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滚筒式生物反应器固态发酵的应用研究
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摘要:
传统方法生产丹贝(大豆发酵食品)都以静态方式进行固态发酵,发酵过程中热质传递受到一定的限制,会产生温度梯度,且气体组分不均匀,从而导致发酵产品质量不稳定。滚筒式生物反应器的应用可以促进热质传递,使物料均匀发酵,且易于实现机械化生产和扩大生产规模。笔者参予设计制造了450L的滚筒式生物反应器,并利用其进行了固态发酵生产丹贝的应用研究。以2株根霉为发酵剂,进行了静态和动态固态发酵的对照试验,并对发酵过程中物料的化学组分和微生物组成进行了检测。结果表明,滚筒式固态发酵产品质量可靠;不同的菌株对搅动有不同的反应,其中微孢子根霉对搅动有适应能力,适用于滚筒固态发酵。
关键词:  固态发酵 滚筒式生物反应器 根霉 丹贝 大豆发酵食品
DOI:
基金项目:
Agitated Solid Substrate Fermentation of Soybeans in a Rotary Drum Bioreactor
Abstract:
Agitated solid substrate fermentation of soybeans to tempe was studied in a Rotary Drum Bioreactor(RDB). Traditionally, tempe is fermented in static layer trays or wrapped packages. Due to heat and mass transfer limitations, gradients of temperature and gas atmosphere will be result in. Agitated fermentation can help to level heat and mass gradients, yielding better homogeneity. For agitated solid substrate fermentation a 450 liter size rotary drum bioreactor was designed and constructed. The comparative effect of static versus agitated solid substrate fermentation using two Rhizopus spp is studied. According to the chemical analyses of substrate modification, it was shown that two Rhizopus spp have different behavior on agitation. Rhizopus microsporus could be used as a starter in agitated solid substrate fermentation.
Key words:  solid substrate fermentation,Rotary Drum Bioreactor,Rhizopus spp