| 摘要: |
| 低聚木糖又名木寡糖，是一种具有多种保健功能的食品添加剂，主要以富含半纤维素的原料经一定的加工方式制得。介绍了采用sp.E-86菌株制备木聚糖酶的详细过程，探讨了发酵时间与木聚糖酶活性以及木聚糖酶液pH值之间的关系，同时提供了一种以玉米芯为原料，采用质量分数为2％的NaOH溶液预处理，通过sp.E-86菌株产木聚糖酶酶解制备低聚木糖的试验方法。考察了不同酶解反应时间条件下低聚木糖产物平均聚合度的变化情况，并用TLC法测定出本研究所得的产物是以木糖、木二糖为主的低聚木糖产品。 |
| 关键词: 玉米芯 木聚糖酶 低聚木糖 酶解 |
| DOI： |
| 修订日期：2000-09-29 |
| 基金项目: |
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| Study on Preparation of Xylooligosacchrides From Corncobs by Enzyme System |
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| Abstract: |
| The detail processing for preparing the xylanase from sp.E 86 strain is investigated. The relationship among the fermentation time to the xylanase activities, and the pH of the xylanase solutions are discusses. A method of preparing xylooligosacchrides from corncobs is introduced. The corncob powder was treated with 2% NaOH solution, and then degraded to xylooligosacchrides by the xylanase of sp E86 strain. The variability of the xylooligosacchrides' degree of polymerization (D.P) under different enzyme reaction times was studied. The TLC results showed that the main components in the products are xylose and xylobiose. |
| Key words: corncob,xylanase,xylooligosacchrides |
