摘要: |
选取不致病的葡萄土壤杆菌(Agrobacterium vitis)E26菌株,以HLB-2和K84菌株做对照,对不同来源的36株根癌菌进行室内及温室条件下的抑菌及防治效果试验,结果表明:室内抑菌检测中,E26的抑菌谱要广于HLB-2和K84,对36株根癌菌中的30株有拮抗作用,抑菌率为83.3%,在温室防治检测中,生防菌与根癌菌1:1混合接种指示植物向日葵 时,E26对85.7%的试菌株有防治效果;与对照生防菌比,尤以防治葡萄根癌病最为有效。菌东研究表明,E26后代菌株与初始菌在产素及生产速度方面均无显著差异,表明E26菌株细菌素的产生是一个比较稳定性的性状。 |
关键词: 生物防治 根癌病 葡萄土壤杆菌 根癌菌 拮抗作用 防效稳定性 |
DOI: |
修订日期:2000-06-27 |
基金项目:北京市自然科学基金资助项目! (6 982 0 15) |
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Preliminary Study on Effectiveness and the Stability of E26 on Controlling Crown Gall Disease |
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Abstract: |
Nonpathogenic strain E26 isolated from the crown gall of grapevines was used in the study for the control effectiveness on crown gall diseases. Plating and greenhouse tests revealed that E26 could control 83.3% and 85.7% of the tested strains respectively, which suggested that E26 was an effective strain to control crown gall, especially for grapevine crown galls. Agrocin assay by transferring strain E26 on plates indicated that agrocin production of E26 is a stable property. |
Key words: biocontrol,crown gall,bacteriocin |