| 摘要: |
| 以１２个苹果砧枯木基因型为试材，建立了扩增酶切片段长度多态性（ＡＦＬＰ）分析体系，对其分析过程中ＤＮＡ提取、双酶切、连接、预扩增、标记和选择性扩增的效果进行了检测。用Ｐ３２Ｍ６４引物构建了供试苹果砧木基因型的ＡＦＬＰ指纹图谱。该指纹图谱拥有扩增条带１０５条，多态性带６７条（占６３．８％），条带清晰可辨，扩增信号强，无背景干扰。讨论了ＡＦＬＰ分析的影响因素。 |
| 关键词: AFLP分析 DNA指纹图谱 苹果 |
| DOI： |
| 修订日期：1999-09-15 |
| 基金项目:国家自然科学基金 !39770 52 3，教育部博士点基金资助项目 |
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| Construction of AFLP Analysis System in Apple |
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| Abstract: |
| AFLP(Amplified Fragment Length Polymorphism)analysis system was established with 12 apple rootstock genotypes( Malus ). The results of several reactions during AFLP analysis have been detected such as DNA extraction,double enzymes restriction,adapter ligation,pre amplification,labeling and selective amplification. The AFLP fingerprintings of 12 apple rootstock genotypes with primer P32M64 have been performed,which had 105 clear and strong amplification bands with bright background. The factors which affect AFLP analysis were discussed. |
| Key words: AFLP system,DNA fingerprinting,apple rootstocks |
