| 摘要: |
| ｇｌｎＢ基因编码的ＰⅠⅠ蛋白在巴西固氮螺菌的固氮过程中起着非常重要的调控作用，而ｇｌｎＺ是与ｇｌｎＢ高度同源的基因，其产物ＰＺ可能也在固氮调控中起作用。本研究用ＰＣＲ法克隆了巴西固氮螺菌Ｙｕ６２ ｇｌｎＢ基因和ｇｌｎＺ基因。ＤＮＡ序列分析表明ｇｌｎＢ和ｇｌｎＺ这２个基因的编码区的长度都为３３６ｂｐ，编码１１２个氨基酸。将巴西固氮螺菌Ｙｕ６２菌株与标准菌株Ｓｐ７的ｇｌｎＢ基因和ｇｌｎＺ基因分别进行比 |
| 关键词: 巴西固氮螺菌 glnB基因 glnZ基因 序列分析 克隆 |
| DOI： |
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| 基金项目:国家 8 6 3高科技计划! (86 3- 10 1- 0 3- 0 4 - 0 2 )资助项目 |
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| Cloning and Sequencing of glnB and glnZ Genes from Azospirillum brasilense |
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| Abstract: |
| The gln B and gln Z genes of Azospirillum brasilense Yu62 are cloned by PCR amplification . DNA analysis shows that the nucleotide sequence of the coding region of gln B gene from A.brasillense Yu62 is same with that of standard A.brasillense Sp7, while the nucleotide sequence of the coding region of gln Z gene from A.brasillense Yu62 is 98.8% identical to that of standard A.brasillense Sp7. There are 4 nucleotide bases that are changed in the coding region of gln Z gene, but the preticted amino acid residues are not changed. The homology of the nucleotide sequences of gln B and gln Z is 73.2%, while the homology of the amino acid sequences encoded by gln Z and gln B is 66.7%. |
| Key words: Azospirillum brasilense Yu62,gln B gene,gln Z gene,PCR,DNA analysis |
