| 摘要: |
| 本文结合反义ＡＣＣ基因的番茄中的导入，就番茄外源基因转化系统建立中的几个问题做了研究。结果表明，转基因番茄的组织培养比普通番茄更复杂和困难，需要对其培养基的激素配比做系统研究，以选择更适合的培养基，抗生素的使用要掌握好剂量，在番茄的子叶上以５０ｍｇ／Ｌ为宜；叶盘法转化番茄子叶时采用的菌液浓度及处理时间对转化也有很大的影响，以农杆菌过夜培养液用ＭＳ液体培养基稀释１０倍，浸泡时间为５ｍｉｎ为宜；转基因 |
| 关键词: 番茄 外源基因 转化 激素 抗生素 |
| DOI： |
|
| 基金项目: |
|
| Tomato Transgenatic System with Ti Plasmid of Agrobacterium tumefaciens |
|
|
|
| Abstract: |
| Tomato transgenetic system was studied with transfer of Antisense ACCase gene by Ti plasmid of Agrobacterium tumefaciens. The results showed that the plant regulators played more important role in tissue culture of tansgenetic plant than than of normal plant. lt was found that Zeatin (1.0 mg/L,) and IAA(0. 5 mg/L,) had great effects on the formation of callus and shooting. The suitable content of kanamycin was 50 mg/L, for selecting transgenetic tissue. The content of Agrobacterium tumefaciens for dipping leaf discs was dilution 10 times with MS liquid medium. Southern blot, RNA blot and morphological properties of plant were detected to identify the transgenetic plant. |
| Key words: tomato,extragene,transfer,plant-regulator,kanamycin |
