| 摘要: |
| 对太湖猪（二花脸）、大约克猪、北京黑猪、五指山猪、香猪、长白猪、杜洛克猪以及太湖猪与7个品种猪的正反交杂交家系线粒体DNAD－loopPCR－RFLP分析，发现猪的线粒体DNAD－loop约为1500bp，长白猪及部分大约克猪与其他猪品种在AccⅠ酶切位点存在多态性；太湖猪、大约克猪的正反交后代的线粒体DNAD－loop在AccⅠ酶切位点存在多态性，其遗传方式遵循母系遗传特性。 |
| 关键词: 猪,线粒体DNA,D－loop,PCR－RFLP,线系遗传 |
| DOI： |
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| 基金项目:自然科学基金!“太湖猪高繁殖性能的遗传基础研究” |
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| PCR-RFLP Analysis of Porcine Mitochondrial DNA D-loop |
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| Abstract: |
| Mitochondrial DNA D-Loops of Taihu (Erhualian),Large White,Beijing Black,Xiang Mini pig, Wuzhishan Mini pig, Landrace,Duroc were amplified and analyzed using 24restriction endonucleases. The molecular size of porcine mitochondrial DNA D-loop amplified was about 1 500 bp, and the cleavage patterns of 24 restriction endonucleases, except Acc1 were identical among all pigs examined, the D-loop digested by Accl was polymorphic that had 150bp and 1 350 bp fragments in all Landrace and parts of Large White while other breeds' D-loop had no cleavage site. the restriction patterns of reciprocal cross of family line between Taihu and Large White showed maternal inheritance. |
| Key words: pig,mitochondrial DNA,D-loop,PCR-RFLP,maternal inheritance, |
