| 摘要: |
| 本文综述了猕猴桃中的ＰＭＥ，ＰＧ在果实软化中的作用，分离提纯，性质和活力测定方法，以及这两种酶的抑制因子等方面的研究进展。ＰＭＥ与猕猴桃果实的软化无直接关系，可能是通过为ＰＧ准备作用底物而起间接的促进软化的作用。 |
| 关键词: 猕猴桃 果胶甲酯酶 抑制因子 PG 果实 |
| DOI： |
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| 基金项目: |
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| A Review of Pectinmethylesterase, Polygalacturonase and Their Inhibitors in Actinidia |
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| Abstract: |
| The functions, isolation &. purifications, properties and activities of PMG, PG and their inhibitors in kiwi fruit Actinidia are reviewed in this paper. There is no direct relationship between the activity of PME and the softening of Actitinia, but PME may produce the substrate for PG so that to enhance the softening process indirectly. The inhibitor of PME in Actinidia is a glucoprotein and appears to be specific for PME. The increase of PG activity is the main factor to promote the distruction of pectin substance and therefore result in the softening of Actinidia after harvest. A specific inhibitor existed in Actinidia fruit that is named actinidin, which inhibits the activity of PG significantly. The activity of actinidin can be triggered by mercaptoethanol and DTT, and also be inhibited by leupeptin and PCMB. |
| Key words: Actinidia,pectinmethylesterase(PME),polygalacturonase(PG),inhibitor |
