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棉铃虫(Helicoverpa amigera Hübner)滞育蛹和非滞育蛹血淋巴某些生化特性的比较研究
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摘要:
试验研究了棉铃虫滞育蛹与非滞育蛹血淋巴在甘油、氨基酸、脂肪酸以及蜕皮激素含量上的不同。结果表明:1)滞育蛹血淋巴17种游离氨基酸的总量比非滞育蛹增加,其中脯氨酸、天冬氨酸、精氨酸、赖氨酸的含量明显高于非滞育蛹,已解除滞育的蛹血淋巴中氨基酸的组成与非滞育蛹仍有所不同,其中丝氨酸、苯丙氨酸、组氨酸的比例明显增加。2)滞育蛹和非滞育蛹血淋巴总脂中的饱和脂肪酸均以棕榈酸为高。不饱和脂肪酸中均以亚油酸含量为高,且滞育蛹的饱和脂肪酸比例高于非滞育蛹。3)血淋巴中甘油的含量两者无显著差异。4)滞育蛹在滞育期血淋巴中20-羟基脱皮酮滴度一直维持在极低水平。当滞育开始解除时,20-羟基脱皮酮的滴度明显上升。
关键词:  棉铃虫,滞育,血淋巴,脱皮激素
DOI:
基金项目:国家自然科学基金
Abstract:
Studies wete carried out to compare the content differences of glycerol, free amino acids, fatty acids and 20 - hydroxy - ecdysone titers of haemolymph in diapausing pupae and non-diapausing pupae of cotton bollworm. The results indicated: 1) The total amout of 17 kind of free amino acids were increased in diapauing pupae against non-dipausing pupae. Of them, the content of PRO. ASP. ARG- LYS. were significantly higher in diapausing pupae than non-diapausing ones. The content of SER. PHE. HIS. of diapauseterminated pupae were still higher than that of non-diapausing pupae. 2)Predominant ingredients of fatty acids were palmitate (saturated fatty acid) and linoleate (unsaturated fatty acid) in both kind of pupae of cotton bollworn, but the ratio of saturated to unsaturated fatty acids of diapausing pupae in the haemolymph lipid was more highter than that of non-diapausing ones. 3)The content of glycerol of pupae in haemolymph had no difference between diapausing and non-diapausing pupae. 4)The very low level of 20-hydroxyecdysone titers in the haemalymph plays an important role of maintaining diapause condition,its prompt rise signals termination of diapause.
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