| 摘要: |
| 玉米赤霉烯酮是植物体内源产生的一类小分子生理活性物质，已证明它与冬性植物的春化作用密切相关。本研究应用亲和层析和SDS-聚丙烯酰胺凝胶电泳技术，对春化的冬小麦幼苗中的玉米赤霉烯酮结合蛋白进行了分离纯化。供试材料为冬小冬品种燕大1817，种子萌发后在4℃一春化4周，提取幼苗的水溶性总蛋白进行亲和层析。层析基质为琼脂糖凝胶Sepharose4B，配体为玉米赤霉烯酮多聚赖氨酸复合物。总蛋白溶液先经过Sepharose4B结合后，再与连有配体的亲和胶进行反应，然后洗去非结合蛋白；改变洗脱条件，进一步洗涤得到的为玉米赤霉烯酮结合蛋白（ZBP）溶液。研究结果表明，在春化的冬小麦幼苗中存在两种玉米赤霉烯酮结合蛋白，它们的分子量分别为25.4kD和22.9kD。 |
| 关键词: 冬小麦 玉米赤霉烯酮结合蛋白 分离 纯化 亲和层析 植物激素 受体 |
| DOI： |
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| 基金项目:国家自然科学基金!39330010 |
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| Isolation and Purification of Zearalenone-Binding Protein from Wheat Seedlings |
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| Abstract: |
| Two kinds of soluble zearalenone-binding proteins (ZBP) in the vernalized seedlings of wheat (Triticum aestivum L. YanDa 1817) were isolated and purified using affinity chromatography and SDS-PAGE. The results indicated that affinity chromatography using immobilized zearalenone coupled to Sepharose 4B via polylysines was very effective for purification of the proteins. The molecular weight of the proteins were estimated to be 25.4 kD and 22. 9 kD by SDS-PAGE. The charateristics of these ZBPs are under study. |
| Key words: zearalenone,binding protein,affinity chromatography,Triticum aestivum L |
