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蜡蚧轮枝菌的发酵培养及其代谢产物对害虫毒杀作用的初步研究
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摘要:
研究表明,蜡蚧轮枝菌(Verticillium lecanii(Zimm.))在2种液体和5种固体培养基中发酵比较,以麦芽汁液体和小米粒固体培养基中的产孢量和分生孢子活力较高。液体发酵5~12 d 的代谢产物粗提物(稀释10倍)对温室白粉虱成虫的毒杀作用,以发酵7 d 的为最好,24 h 校正死亡率为50.18%,但与发酵5 d 的(34.18%)无显著差异(P<0.05)。代谢产物粗提物对棉铃虫、麦长管蚜的触杀作用较好,对瓜蚜次之,对桃蚜最差。如试验24 h,稀释5倍的代谢产物杀虫校正死亡率,麦长管蚜66.7%、棉铃虫66.7%、瓜蚜48.8%、桃蚜23.4%。稀释2倍时,24 h 瓜蚜的校正死亡率为78.8%,略高于氰戊菊酯(7768mg·L-1)的毒杀作用(77.2%)。说明该菌代谢产物中具有杀虫活性物质,值得进一步研究利用。
关键词:  蜡蚧轮枝菌  发酵  代谢产物粗提物  温室白粉虱  棉铃虫  蚜虫  
DOI:
投稿时间:1995-01-12
基金项目:
A Study on Ferment Cultrues of Verticillium lecanii and the Efficacy of the Fungus Metabolites on Insect Pests
Abstract:
Research results showed that sporulation yields and vitality of conidia were higher in fer- ments of malt extract liquid and millet grain solid than in media of other liquids and solids based on the comparison of ferments of Verticillium lecanii(Zimm.).The efficacy of crude extract(10 dilution times) of the fungus metabolites,fermented in liquid for 7 days,on adults of Trialeurodes vaporariorum West- wood showed best among fermented in liquid for 5 to 12 days.Adjusted mortality of the adults in 24 hours by ...
Key words:  Verticillium lecanti  lerment  crude extract ot metabolite  Trialeurodes vaporariorum  Helicoverpa armigera  aphid