| 摘要: |
| 本实验以042B 的 DNA 转化 ROG31细胞,在以0.2 mol/L NaCl 为选择标记的YMA 培养基上,获得37个耐盐的菌落,纯化后又经30代的转管移植,有8株转化子获得了稳定的耐盐性,再将这8株转化子回接红豆草植株,得到1株耐0.4 mol/L NaCl 和固氮能力较好的红豆草根瘤菌 ROt6。供体菌042B 和转化子 ROt1和 ROt6在0.3 mol/L NaCl 培养基中生长时,细胞内积累大量谷氨酸。 |
| 关键词: DNA 转化 耐盐性 红豆草根瘤菌 |
| DOI： |
| 投稿时间：1992-09-01 |
| 基金项目: |
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| The Improvement of Salt Tolerance and Symbiotic Characteristics of Sainfoin Rhizobia |
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| Abstract: |
| Rhizobium strain ROG31 isolated from the root nodules of sainfoin(Onobrychis viciaefolia)in Gansu province is sensitive to 0.1 tool/L NaCl and less efficient in nitrogen fixation. Alfalfa rhizobium 042B can grow in YM liquid medium containing 0.8 mol/L NaCl,and has high activi- ty of symbiotic nitrogen fixation.Thirty seven transformants were obtained by transforming the DNA from strain 042B into the cells of strain ROG31 and selected on YMA medium with 0.2 mol/L NaCl as seleceive marker.However,only eight ... |
| Key words: DNA transformation salt tolerance sainfoin rhizobia |
