| 摘要: |
| 本试验选用 SD 系大鼠,用常规阴道抹片法鉴定发情。采用免疫组织化学的过氧化物酶—抗过氧化物酶复合物(PAP)法,进行雌激素受体(ER)定位。在子宫的内膜固有层、肌层和浆膜层,发现抗雌二醇抗体的免疫反应阳性细胞,而在内膜上皮细胞内无阳性染色。这种阳性染色可以被乙烯酚所阻断,不加抗雌二醇抗体的结果使染色呈阴性。发情周期4个不同时期的子宫组织中免疫反应阳性细胞表现出量的变化,以发情间期为基础,发情前期较多,发情期和发情后期依次减少,其动态变化与前人报道发情周期中雌二醇在血浆中的浓度变化是对应的。并发现发情周期中子宫组织的肥大细胞与雌激素受体的分布和变化有着相似的特点。 |
| 关键词: 大鼠 发情周期 雌激素受体 子宫 |
| DOI： |
| 投稿时间：1990-12-13 |
| 基金项目: |
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| Preliminary Study on the Localization of Estrogen Receptors in Rat Uterus during Estrous Cycle |
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| Abstract: |
| With improved peroxidase-antiperoxidase complex method,loca- lization of estrogen receptors in tissues of rat uterus has been made The result was,at the four stages of rat estrous cycle,there were positive immunos- taining cells in the endometria,muscularis and serosa of uterus,but not in the endometrium epithelia.This positive staining could be broken down by using DES instead of estrodiol.Also there were quantitative variations in staining cells among the four stages,its variation was in accordance with t... |
| Key words: rat estrous cycleestrogen receptors uterus |
