| 摘要: |
| 研究了一种新的根据活体外人工瘤胃产气量与游离 NH3-N 量估测反刍动物饲料蛋白质瘤胃降解率的方法。饲料样本与瘤胃液在活体外培养6 h,其产气量与组入瘤胃微生物蛋白质中的NH_3-N 量呈高度正相关(r>0.99),故产气量可用于准确度量组入微生物蛋白质中的 NH3-N量。以饲料样本的游离 NH3-N 量与根据产气量估测的组入 NH3-N 量之和占其总 N 量的比例,作为其瘤胃蛋白质降解率的度量。本研究方法简单、快速、成本低,所测结果与体内法结果具有高度相关性(r>0.97)。 |
| 关键词: 产气量 游离 NH3-N 量 活体外法 瘤胃蛋白质降解率 |
| DOI： |
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| A NEW IN VITRO PROCEDURE FOR ESTIMATION OF RUMINAL PROTEIN DEGRADATION |
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| Abstract: |
| A new in vitro procedure for estimation of ruminalprotein degrada- tion was established by measurements of 6-hour gas production and free NH_3-N concentration of ruminant feedstuffs when they were incubated with rumen liquor-buffer solution.A linear regression between NH_3-N incorporated into microbial proteins(y,mg)and gas production(x,mL)was found to be highly significant(r>0.99),indicating that the gas production of a feed can be regar- ded as a more accurate estimate of the incorporated NH_3-N.The free ... |
| Key words: gas production free NH3-N in vitro ruminal protein degradation |
