| 摘要: |
| 构建了β-葡聚糖酶表达载体 PSGI。转化枯草杆菌 SO113后,可高效表达β-葡聚糖酶,并分泌到培养基中。其酶活性约为无表达质粒的枯草杆菌的十倍。 |
| 关键词: β-葡聚糖酶基因 表达载体的构建 转化枯草杆菌 SO113 高效表达 |
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| Abstract: |
| An expression vector of Beta-glucanase gene p~(Sa1) was construc- ted.High expression level of Beta-glucanase was obtained after SO113,a B. subtilis strain,was transformed with the vector and the enzyme was secreted into the medium.The enzymetic activity was found to be tenfold as the control- led bacteria without the vector.Plasmid P~(SC1) may be used as a general expres- sion vector for the expression of other genes in B.subtilis. |
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