| 摘要: |
| 本试验对绵羊垂体前叶催乳素进行了提纯和生物学、生物化学及免疫学方面的鉴定.516g 羊全垂体经硫酸铵、乙醇等的逐级提取,最后经 DEAE-纤维素柱层析得到催乳素纯品450mg.用鸽嗉囊局部皮内微量注射法测得其生物学活性为27国际单位(27IU/mg)。PAG 电泳表明有三条带。SDS 电泳测得其分子量为23000。IEFP 电泳表明平均等电点为5.85。用 Dansyl-Cl 法测定其 N-末端氨基酸,只显出苏氨酸一点。表明提纯的催乳素是高纯的。用该催乳素免疫家兔,九周后用琼脂双扩散法测抗血清,其效价最高达1:32,说明该纯化品具有很强的免疫原件. |
| 关键词: 羊催乳素 提纯 鉴定 抗血清 |
| DOI： |
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| ISOLATION AND CHARACTERIZATION OF OVINE PITUITARY PROLACTIN AND PRODUCTION OF ITS ANTISERUM |
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| Abstract: |
| Through a series of extraction and precipitation with ammonium sulfate and chro- matography on DEAE-cellulose,we achieved 450rag purified prolactin from 516g sheep whole frozen pituitary.The yield was 0.87g per kilogram frozen pituitary. With the method of local micro-introdermal injection over the pigeon crop sac, we deduced that our sample possessed an activity of 27IU/per rag.On the polycry- lamide gel electrophoresis,the sample ran into three distinct bands.The SDS- PAG electrophoresis showed that the m... |
| Key words: sheep prolactin isolation characterization antisera |
