| 摘要: |
| 显微光度计可以直接测量单细胞内核酸、蛋白质和酶的含量,每条染色体的 DNA 量和带数以及免疫反应中结合抗体的定量等。我们用 Univar 显微光度计比较了吸收扫描法和荧光法的效果。实验测定了小麦和大麦根尖细胞、鸡血细胞,镰刀菌孢子和酵母菌不同生长阶段的细胞。结果表明吸收扫描法测定小麦六倍体、四倍体和二倍体 DNA 含量,其比值为3.2:2.1:1。荧光测定酵母菌子囊孢子 DNA 含量(任意值)为462.61,营养细胞为914.38,两个子囊孢子核结合后为925.08。对大细胞(植物)两种方法效果都好。对小细胞(微生物)荧光法较吸收扫描法更为稳定。 |
| 关键词: 显微分光光度计 吸收扫描法 荧光分析 DAN 含量 |
| DOI： |
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| 基金项目: |
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| MICROSPECTROPHOTOMETRIC MEASUREMENT OF DNA CONTENTS OF DIFFERENT CELLS |
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| Abstract: |
| Microspectrophotomety is used to quantify cytochemical substances,viz the amount of nuclear DNA,protein,enzymes and binding antibodies in the indi- vidual cell.Absorption scanning and fluorescence methods were compared for their abilities to measure nuclear DNA content of the root tip cells of wheat and barley,conidia of Fusarium and the yeast spores from differerent stages of growth.Using scanning absorption photometry the ratio of nuclear DNA of sex- tuploid,tetraploid and diploid in wheat cultivars was d... |
| Key words: microspectrophotometer absorption scanning method fluorimetric analysis DNA content |
