| 摘要: |
| 巴西日圆线虫(Nippostrongylus brasiliensis)广泛地用作慢性寄生线虫病的动物模型,特剐是普遍地应用于宿主与寄生虫关系以及寄生虫免疫学的研究(Jacobson,1975,1982)。以往学者们(Haley,1962;Croll,1977等)均用尸体剖检后在组织中找虫的方法研究本虫在大鼠体内的分布及移行。Georgi(1982,1983)曾用同位素标记技术和放射自显术,成功地研究了血吸虫和捻转血矛线虫在动物体内的分布。我们首次试用了本法研究巴西日圆线虫在小鼠体内的分布和移行,它为进行巴西日圆线虫的免疫学研究,以及药物筛选等提供可靠的方法。 |
| 关键词: 药物筛选 放射 同位素标记 寄生虫 感染后 移行途径 三期幼虫 巴西日圆线虫 小鼠 测定 |
| DOI： |
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| RADIOLABELING OF INFECTIVE LARVAE OF NIPPOSTRONGYLUS BRASILIENSIS WITH ~(75)SE-METHIOINE AND DETECTION OF LARVAE MIGRATION IN TISSUES OF MOUSE BY AUTORADIOGRAPHY |
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| Abstract: |
| Nippostrongylus brasiliensis is a widely-used model of chronic nema- todiasis.It is used frequently in studies of host-parasite relations,espe- cially in immunology.Through admixture of ~(75)se-rnethionine and fecal se- diment was necessary to obtain approximately normal distribution and low variance of individual larval radioactivities.Radiolabeling of infective la- rvae of N.brasiliensis was successful(121.7 cpm/larvae).Autoradio- graphy was a very useful method for studying migration of larvae in vi- va.... |
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