| 摘要: |
| 由甘蓝(Brassica oleracea)和芜菁(Brassica rapa)黄化小苗子叶和下胚轴分离出大量有活力的原生质体,并成功地进行了培养。30小时后发生第一次分裂。下胚轴原生质体分裂频率明显高于子叶原生质体。除芜菁子叶原生质体外,其余材料的原生质体均能持续分裂,20天后形成肉眼可见的愈伤组织。在 MS 培养基上,来源于甘蓝子叶原生质体的愈伤组织分化出根,其余的未见分化现象。 |
| 关键词: 甘蓝 小愈伤组织 原生质体培养 叶原生质体 芸苔属 固体培养基 下胚轴原生质体 培养液 子叶 分裂频率 |
| DOI： |
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| ISOLATION AND CULTURE OF PROTOPLASTS OF TWO BRASSICA SPECIES. |
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| Abstract: |
| A Iarge number of viable protoplasts were isolated by enzyme treat- ment from cotyledons and hypocotyls of etiolated seedlings of Brassica oleracea and Brassica rapa,and were cultured successfully.The first division was observed after 30 hours.The division frequency of hypoco- tyl protoplasts was obviously higher than that of cotyledon protoplasts. Cell division in all cases except cotyledon protoplasts of Brassica rapa continued,and after 20 days of culture small calli were visible. Rhizogenesis was observ... |
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