| 摘要: |
| 1.5-二磷酸核酮糖羧化酶(RuBPCase)是光合作用碳循环中的关键晦,是研究植物分子遗传的重要材料,本文对 RuBPCase 进行了分离、提纯和结晶,并对其结晶条件进行了探索, 同时对 RuBPCase 晶体进行了活性测定、氨基酸组分分析、紫外线测定和电泳鉴定。 |
| 关键词: 分离提纯 电泳鉴定 结晶条件 植物分子 组分分析 氨基酸 活性测定 烟草 二磷酸核酮糖羧化酶 缓冲液 |
| DOI： |
|
| 基金项目: |
|
| SEPARATION,PURIFICATION AND CRYSTALLIZATION OF RIBULOSE BIPHOSPHATE CARBOXYLAE FROM TOBACCO LEAVES |
|
|
|
| Abstract: |
| Ribulose biphosphate carboxylase from Tobacco leaves has been separated and purified and crystals have been obtained. The identification of the crystalline material with Ribulose bipho- sphate carboxylase was carried out by analytical methods,ultra-violet absorption,isotopic ~(14)C and so on. |
| Key words: |
