| 摘要: |
| 从自然界中分离得到的玉米赤霉菌 G.zeae BAU-z8菌系只具有在固体培养基中合成玉米赤霉烯酮的能力。经过亚硝基胍和紫外线对 BAU-z8的诱变处理后,再经筛选和分离纯化,得到了具有在液体常温条件下生成玉米赤霉烯酮的突变株D4-21,并对其生成玉米赤霉烯酮的条件进行了研究。试验表明:培养基的碳源、氮源种类;碳、氮、磷的浓度及 C/N 值;初始 pH 值、培养温度、通气量等因素都对玉米赤霉烯酮产量有很大影响。通过试验,确定了摇瓶发酵培养基成份的最佳组合以及最适的培养条件为:葡萄糖18%、尿素0.27%、K2HPO4 0.05%、酵母膏0.10%、MgSO4 0.25%、KCl 0.025%、pH5.4—5.5(自然)、装量:120ml/500ml 摇瓶、温度:21—24℃,经摇床震荡培养2周,产量可达2—2.2g/l。 |
| 关键词: 摇瓶发酵 培养温度 分离纯化 最佳组合 突变株 常温合成 合成条件 酵母膏培养基 玉米赤霉烯酮 |
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| PRODUCTION OF ZEARALENONE BY GIBBRELLA ZEAE MUTANT D_(4-21) IN LIQUID MEDIA |
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| Abstract: |
| A mutant of Gibberella zeae BAU_(_z8)was obtained through the treatment with NTG and UV light.BAU_(_z8)was isolated from corn grown in the suburb fields of Beijing by Li et al(2),which produced zearalenone only in solid media.The mutant D_(4-21),however,can produce zearalenone in liquid media under room temperature. The effects of the concentration of C.N.P.and C/N ratio,the initial pH value of the medium,the temperature and aeration on production of zearalenone were tested.It was found that the best yield ... |
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