| 摘要: |
| 自北京郊区的玉米根系分离出玉62菌株,用乙炔还原法测定其固氮酶活性比来自巴西的 Sp81高60%。自高梁根系分离出高63菌株。其形态、生理等特征都与玉62相似,两菌株均属巴西固氮螺菌(Azospirillum brasilense)以玉62为出发菌株,经亚硝基胍处理后,曾得到一些能在含有0.2%NH_4Ac 和KCN(或 NaN_3)的培养基上生长并有较高固氮酶活性的抗氮菌株。如127—6菌株在0.2%NH_4Ac 培养基上的固氮酶活性比玉62高3.5倍。但这种特性不易保持,经过一段时间,其酶活性下降。曾作几个菌株接种夏玉米的小区田间试验。虽然某些菌株对产量有一定的效果,但统计学上差异不显著。 |
| 关键词: 固氮螺菌属 固氮酶活性 田间试验 半固体培养基 巴西固氮螺菌 菌株 夏玉米 接种试验 北京郊区 玉米根系 |
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| STUDY OF AZOSPIRILLUM IN BEIJING DISTRICT |
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| Abstract: |
| A strain of Azospirillum,Yu-62,was isolated from the roots of corn grown in the suburb field of Beijing.It's nitrogenase activity as tested by C_2H_2 reduction method was 60% higher than that of the Azo- spirillum brasilense Sp 81 which was isolated by D bereiner in Brazil. Another strain,Gao-63,isolated from the roots of sorghum is similar to Yu-62 in morphological and physiological characters and both belong to AzospirilIum brasilense. Some NH_4~+ tolerant mutants such as 127-6,43 and 55 were obtained by ... |
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