| 摘要: |
| 本文介绍了应用不同性能,不同型号的分光光度计正确测定叶绿素的几种主要方法。包括双波长联立方程法,双波长差分法及等吸收点法。其中探讨了波长偏差,单色光带宽,分光光度计性能,溶剂和不同的提取方法以及叶绿素提取液的状态等各种条件对测定叶绿素含量的影响。介绍了消除某些影响的校正方法供读者参考。 |
| 关键词: 双波长差分 联立方程法 双波长分光光度计 提取液 分光光度计测定 叶绿素含量 消光度 消光系数 |
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| STUDIES ON DETERMINATION CHLOROPHYLLS A AND B |
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| Abstract: |
| This is a report on the methods which we have used to determine the content of chlorophills a and b on spectrophotometers of diffdrent types and with different qualities.The methods are as follows: 1.Solving simultaneous equations obtained from doublewave length spectrophotometry; 2.Differentil double-wavelength spectrophotometry; 3.Equal-absorptance method. A disscusion on the wave-length error,band-width of the mono- chromatic light and other characteristics of these spectrophotometers are given. The infl... |
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